Electrochemical DNA-based Biosensor Research Articles

Identification of Influential Factors in the Development of Modified Screen-printed Carbon Electrode in the DNA-based Electrochemical Biosensor Using the Design of Experiment

Identification of Influential Factors in the Development of Modified Screen-printed Carbon Electrode in the DNA-based Electrochemical Biosensor Using the Design of Experiment

Assessment of the Antioxidant Capacity of Commercial Coffee Using Conventional Optical and Chromatographic Methods and an Innovative Electrochemical DNA-Based Biosensor.

As one of the most popular beverages in the world, coffee is a rich source of non-enzymatic bioactive compounds with antioxidant capacity. In this study, twelve commercial coffee beverages found in local Portuguese markets were assessed to determine their total phenolic and flavonoid contents, as well as their antioxidant capacity, by conventional optical procedures, namely, ferric reducing antioxidant power and DPPH-radical scavenging assay, and non-conventional procedures such as a homemade DNA-based biosensor against two reactive radicals: HO• and H2O2. The innovative DNA-based biosensor comprised an adenine-rich oligonucleotide adsorbed onto a carbon paste electrode. This method detects the different peak intensities generated by square-wave voltammetry based on the partial damage to the adenine layer adsorbed on the electrode surface by the free radicals in the presence/absence of antioxidants. The DNA-based biosensor against H2O2 presented a higher DNA layer protection compared with HO• in the presence of the reference gallic acid. Additionally, the phenolic profiles of the twelve coffee samples were assessed by HPLC-DAD, and the main contributors to the exhibited antioxidant capacity properties were caffeine, and chlorogenic, protocatechuic, neochlorogenic and gallic acids. The DNA-based sensor used provides reliable and fast measurements of antioxidant capacity, and is also cheap and easy to construct.

Branched DNA-Based Electrochemical Biosensor for Sensitive Nucleic Acids Analysis with Gold Nanoparticles as Amplifier.

A branched DNA-based electrochemical biosensor was designed to sensitively detect specific nucleic acids. On this platform, novel a branched DNA with three sticky ends could be used as a biosensor to sensitively and specifically detect nucleic acids. Meanwhile, we also employed branched DNA-modified AuNPs as a signal amplifier to further improve the sensitivity. Branched DNA sensors, target DNA, and DNA-modified AuNPs formed a sandwich structure to produce an electronic signal for target DNA detection. The reaction primarily involved DNA hybridization without bulky thermal cyclers and enzymes. We proved that the hybridization reaction easily occurred under different conditions, such as the NaCl concentration, reaction time, pH, and temperature, except for a pH lower than 4. The limit of detection could go as low as 0.09 pM (S/N = 3) with excellent specificity and selectivity. There was a correlation curve relationship between the peak current and the logarithm of the target DNA concentration (0.10 pM to 10 nM). The correlation coefficient reached 0.987. The electrochemical platform enables a branched DNA nanostructure to determine nucleic acids for disease diagnosis.

Rapid and Quantitative Detection of Lung Cancer Biomarker ENOX2 Using a Novel Aptamer in an Electrochemical DNA-Based (E-DNA) Biosensor.

To overcome early cancer detection challenges, diagnostic tools enabling more sensitive, rapid, and noninvasive detection are necessary. An attractive cancer target for diagnostic blood tests is human Ecto-NOX disulfide-thiol exchanger 2 (ENOX2), expressed in most human cancer types and regularly shed into blood sera. Here, we developed an electrochemical DNA-based (E-DNA) biosensor that rapidly detects physiologically relevant levels of ENOX2. To identify ENOX2-binding aptamers that could potentially be used in a biosensor, recombinantly expressed ENOX2 was used as a binding target in an oligonucleotide library pull-down that generated a highly enriched ENOX2-binding aptamer. This candidate aptamer sensitively bound ENOX2 via gel mobility shift assays. To enable this aptamer to function in an ENOX2 E-DNA biosensor, the aptamer sequence was modified to adopt two conformations, one capable of ENOX2 binding, and one with disrupted ENOX2 binding. Upon ENOX2 introduction, a conformational shift to the ENOX2 binding state resulted in changed dynamics of a redox reporter molecule, which generated a rapid, significant, and target-specific electrical current readout change. ENOX2 biosensor sensitivity was at or below the diagnostic range. The ENOX2 E-DNA biosensor design presented here may enable the development of more sensitive, rapid, diagnostic tools for early cancer detection.

Development of an electrochemical DNA-based biosensor for the detection of the cardiovascular pharmacogenetic-altering SNP CYP2C9*3

Cardiovascular diseases are among the major causes of mortality and morbidity. Warfarin is often prescribed for these disorders, an anticoagulant with inter and intra-dosage variability dose required to achieve the target international normalized ratio. Warfarin presents a narrow therapeutic index, and due to its variability, it can often be associated with the risk of hemorrhage, or in other patients, thromboembolism. Single-nucleotide polymorphisms are included in the causes that contribute to this variability. The Cytochrome P450 (CYP) 2C9*3 genetic polymorphism modifies its enzymatic activity, and hence warfarin's plasmatic concentration. Thus, the need for a selective, rapid, low-cost, and real-time detection device is crucial before prescribing warfarin. In this work, a disposable electrochemical DNA-based biosensor capable of detecting CYP2C9*3 polymorphism was developed. By analyzing genomic databases, two specific 78 base pairs DNA probes; one with the wild-type adenine (Target-A) and another with the cytosine (Target-C) single-nucleotide genetic variation were designed. The biosensor implied the immobilization on screen-printed gold electrodes of a self-assembled monolayer composed by mercaptohexanol and a linear CYP2C9*3 DNA-capture probe. To improve the selectivity and avoid secondary structures a sandwich format of the CYP2C9*3 allele was designed using complementary fluorescein isothiocyanate-labeled signaling DNA probe and enzymatic amplification of the electrochemical signal. Chronoamperometric measurements were performed at a range of 0.015–1.00 nM for both DNA targets achieving limit of detection of 42 p.m. The developed DNA-based biosensor was able to discriminate between the two synthetic target DNA targets, as well as the targeted denatured genomic DNA, extracted from volunteers genotyped as non-variant homozygous (A/A) and heterozygous (A/C) of the CYP2C9*3 polymorphism.

Surface modification of a screen-printed electrode with a flower-like nanostructure to fabricate a guanine DNA-based electrochemical biosensor to determine the anticancer drug pemigatinib.

The present study developed a DNA biosensor to determine pemigatinib for the first time. Three-dimensional carnation flower-like Eu3+:β-MnO2 nanostructures (3D CF-L Eu3+:β-MnO2 NSs) and a screen-printed electrode (SPE) modified with polyaniline (PA) were employed. The double-stranded DNA was also immobilized completely on the PA/3D CF-L Eu3+:β-MnO2 NSs/SPE. Then, electrochemical techniques were used for characterizing the modified electrode. After that, the interaction between pemigatinib and DNA was shown by a reduction in the oxidation current of guanine using differential pulse voltammetry (DPV). According to the analysis, the dynamic range of pemigatinib was between 0.001 and 180.0 μM, indicating the new electrode has a low limit of detection (LOD = 0.23 nM) for pemigatinib. Afterwards, pemigatinib in real samples was measured using the PA/3D CF-L Eu3+:β-MnO2 NSs/SPE loaded with ds-DNA. The proposed DNA biosensor showed good selectivity toward pemigatinib in the presence of other interference analytes, such as other ions, structurally related pharmaceuticals, and plasma proteins. In addition, the interaction site of pemigatinib with DNA was predicted by molecular docking, which showed the interaction of pemigatinib with the guanine bases of DNA through a groove binding mode. Finally, we employed the t-test to verify the capability of the ds-DNA/PA/3D CF-L Eu3+:β-MnO2 NSs/SPE for analyzing pemigatinib in real samples.

Abstract 1735: Electrochemical DNA-based biosensors for Bacterial Pathogens: Aptamer discovery, refinement, and adaptation

Abstract 1735: Electrochemical DNA-based biosensors for Bacterial Pathogens: Aptamer discovery, refinement, and adaptation

An ultrasensitive electrochemical DNA biosensor for monitoring Human papillomavirus-16 (HPV-16) using graphene oxide/Ag/Au nano-biohybrids

A DNA-based electrochemical biosensor has been developed herein for the detection of Human papillomavirus-16 (HPV-16). HPV-16 is a double-stranded, non-enveloped, epitheliotropic DNA virus which responsible for cervical cancer. In this proposed biosensor, an indium tin oxide (ITO) coated glass electrode was modified for sensing HPV-16 using graphene oxide and silver coated gold nanoparticles. Subsequently, HPV-16 specific DNA probes were immobilized on a modified ITO surface. The synthesized nanocomposites were characterized by FE-SEM and UV-VIS spectroscopy techniques. Electrochemical characterization was performed by using cyclic voltammetry and electrochemical Impedance Spectroscopy methods. The hybridization between the probe and target DNA was analyzed by a reduction in current, mediated by methylene blue. The biosensor showed a qualitative inequity between the probe and target HPV-16 DNA. The developed biosensor showed high sensitivity as 0.54 mA/aM for the detection of HPV-16. In a linear range of 100 aM to 1 μM with 100 aM LOD, the proposed biosensor exhibited excellent performance with the rapid diagnosis. Thus, the results indicate that the developed HPV DNA biosensor shows good consistency with the present approaches and opens new opportunities for developing point-of-care devices. The diagnosis of HPV-16 infection in its early stage may also be possible with this detection system.

Laser-Induced graphene electrodes for highly sensitive detection of DNA hybridization via consecutive cytosines (polyC)-DNA-based electrochemical biosensors

Numerous carbon-based biosensors issued mechanical exfoliation, epitaxial growth, reduced graphene oxide, and chemical vapor deposition have been investigated for highly sensitive and specific detection of DNA. As a promising route for designing electrochemical biosensor-based flexible substrates, the laser-induced graphene technique, which provides a cheap, technologically simple, and highly robust sensing platform, has been widely adopted. However, DNA-based biosensors' efficiency is strongly dependent on how DNA probes are tethered to the nanomaterials. In view of this, poly-cytosine (poly-C) DNA has shown outstanding adsorption to multiple inorganic nanomaterials, including gold (Au), zinc oxide (ZnO), tungsten disulfide (WS2), graphene oxide (GO), and graphene. In this work, a poly C(15)-tailed diblock DNA probe is used to anchor to carbonized working electrode issued laser-induced method. Meanwhile, the second block modified with ferrocene (Fc) derivatives is lifted at the surface for DNA sequence recognition. Following this strategy, the developed biosensor leads to a limit of detection (LOD) of 57 fM, which was superior or comparable to some previously reported methods. Moreover, the proposed electrochemical DNA biosensor exhibits high specificity in differentiating the complementary DNA from non-complementary DNA (ncDNA), and mismatched DNAs (MM-DNA) sequences. Finally, the easily constructed laser-induced graphene electrode biosensor showed an ability to detect DNA in human serum as a complex environment, making our approach a promising avenue for disease diagnosis.

Design and Production of DNA-Based Electrochemical and Biological Biosensors for the Detection and Measurement of Gabapentin Medication in Clinical Specimens

Therapeutic Drug Monitoring (TDM) is the clinical practice of measuring specific drugs at designated intervals to maintain a constant concentration in a patient’s bloodstream, thereby optimizing individual dosage regimens. Hence, the extant study was conducted to design and produce an electrochemical and biological biosensor to detect and measure Gabapentin medication. The present study was a laboratory-applied intervention, which used a pencil graphite electrode modified with α-Fe2O3 and MOS2 nanostructures in one step and DNA in the second address layer, by using Differential Pulse Voltammetry (DPV) electrochemical sensing techniques to detect Gabapentin medication in clinical specimens such as serum, plasma, and urine. The functional mechanism of the biosensor is designed in such a way that the effect of interaction between drug and DNA leads to drug detection, and with increasing concentration of Gabapentin analyte, the amount of visible current by DNA is reduced. Significantly, this reduction in current after reaction with Gabapentin can be demonstrated using the Differential Pulse Voltammogram (DPV) curve. The designed biosensor provided some features, including inexpensiveness, simple and fast sensor biodegradation process, appropriate stability of sensor, low Limit of Detection (LOD), extensive linearity range, ease of application without requiring laboratory technician, and selectivity of biosensor materials. Electrochemical and biological biosensors have some advantages rather than the common analytical methods; hence, these biosensors can be used widely in the future. High selectivity, low cost of production and low energy consumption, accuracy and precision, short response time, adaptability, simple preparedness, minimization capability, high-speed data collecting, low LOD, minor operational volume, and robust measurement. On the other hand, biosensors have received great attention over recent years due to their continuous application, the high selectivity of biological compounds, such as enzymes and antibodies, and the ability to measure nonpolar compounds.

Electrochemical DNA Biosensor to Detect Glycopeptidolipids of Nontuberculous Mycobacteria

In many regions, nontuberculous Mycobacteria (NTM) pulmonary infections have outnumbered tuberculosis infections over the past 30 years, yet NTM infections are frequently misdiagnosed. One critical challenge is that the current “gold standard” for NTM diagnosis involves a weeks‐to‐month long microbiological culture‐based method. To provide a more efficient diagnostic method, we have developed a novel electrochemical DNA‐based (E‐DNA) biosensor to detect NTM. Our E‐DNA biosensor targets a mycobacterial glycopeptidolipid (GPL) found on the exterior cell wall of NTM, which demonstrates high exclusivity for NTM. To create the E‐DNA biosensor, we successfully generated a GPL‐specific single‐stranded DNA aptamer through a modified SELEX (systematic evolution of ligands by exponential enrichment) process followed by high throughput sequencing to quantify potential aptamer sequences. The putative aptamers were then synthesized and screened via fluorescence, with the best candidate aptamer displaying a dissociation constant in the low nanomolar range (KDapp = 0.73 nM). Computational analysis was used to predict secondary and tertiary structure of the aptamer, allowing optimization of that aptamer sequence to find the minimum binding domain and engineer in a two‐state folding mechanism. The aptamer will then be synthesized with a 5’ thiol linkage to facilitate attachment to a gold electrode along with an internal redox reporter (methylene blue). This will enable an electrochemical interrogation method wherein changes in the peak current in square wave voltametric analysis correlate to a conformational rearrangement of the aptamer upon binding to GPL. In future studies, this E‐DNA biosensor will be verified against NTM‐positive patient samples. Ultimately, this approach should provide a rapid, point‐of‐care diagnostic method that eliminates the current lengthy culturing requirements to shorten the duration between the onset of symptoms and correctly targeted treatment of patients infected with NTM.

Development of an Electrochemical, DNA‐Based Biosensor to the Cancer Biomarker ENOX2

One challenge to the identification of cancerous malignancies is the ability to detect cancer‐specific antigens in a rapid, reliable, and accessible manner. Human Ecto‐NOX Disulfide‐Thiol Exchanger 2 (ENOX2) is a cell‐surface metalocatalyst frequently expressed on virtually all major human cancer types and is critical for cell maturation. Importantly, ENOX2 regularly sheds into the sera, making it an attractive target for diagnostic blood testing. As a strategy for diagnostics focused on utilizing these shed ENOX‐2 particles, we here demonstrate an electrochemical DNA‐based (E‐DNA) biosensor directed against ENOX‐2 in solution. To do so, we first exploited a modified SELEX (systematic evolution of ligands by exponential enrichment) protocol to generate aptamers against ENOX2 recombinantly expressed in E. coli. A pool of candidate aptamers was identified by high‐throughput sequencing, and gel mobility shift assays were used to characterize the best candidate aptamers and classified those with the highest affinity for ENOX2. The leading aptamer sequence was used as the core of our E‐DNA biosensor, wherein changes in DNA conformation upon target binding result in changed dynamics of an appended redox reporter molecule, ultimately generating a rapid and quantitative change in the current of the electrochemical response when interrogated via voltametric methods such as square‐wave voltammetry. Our sensor displays robust response to ENOX2 in solution, and may allow accelerated, dose‐responsive readout for the presence of ENOX2 in both buffer and blood serum.

An Electrochemical Biosensor for Detection of P.69 Pertactin Associated with B. pertussis

Whooping cough caused by Bordetella pertussis can cause serious and prolonged health effects and is especially deadly in infants. Early treatment can significantly reduce the duration of illness and lead to better outcomes, but current diagnostic procedures need a relatively large patient sample and have an extended wait period for testing results. There is thus a pressing need for quick and accurate diagnosis. In response, we are developing an electrochemical DNA‐based (E‐DNA) biosensor for the rapid detection of B. pertussis. Our biosensor utilizes a DNA aptamer that targets P.69 pertactin, a well‐known adhesion factor in the outer membrane of B. pertussis. To generate this aptamer, we first obtained purified P.69 pertactin and biotinylated it. Biotinylation extent was measured via HABA assay and showed ~ 42 biotin molecules per P.69 pertactin molecule. This biotinylated protein was used for selection in a modified SELEX (systematic evolution of ligands by exponential enrichment) process, followed by high‐throughput sequencing. That analysis discovered six aptamer sequences with potential strong binding for P.69 pertactin. Potential aptamers were synthesized, and binding was confirmed via fluorescence anisotropy and gel shift assays. These techniques identified one aptamer sequence as having strong, low‐nanomolar binding affinity for P.69 pertactin. This aptamer was then computationally analyzed for likely secondary and tertiary structures, allowing identification of the minimum binding unit, and then was modified to generate a two‐state binding system. This modified aptamer sequence serves as the basis for an electrode‐tethered E‐DNA biosensor. This design will be verified against purified P.69 pertactin standards as well as B. pertussis isolates to demonstrate its potential as a rapid and accurate diagnostic biosensor for whooping cough infections.

Designing a novel DNA-based electrochemical biosensor to determine of Ba2+ ions both selectively and sensitively

This work describes a novel electrochemical biosensor based on G-quadruplex (G4) DNA for the sensitive and selective detection of Ba2+ ions using a Carbon Paste Electrode modified with Ag nanoparticles incorporated in reduced Graphene Oxide via a simple wet chemical method (Ag-rGO/CPE), in the presence of carmoisine (CA) as a new electrochemical indicator. The peak current of CA increased with increasing Ba2+ ions concentration and the DNA-based sensor showed linear ranges of 0.06–0.80 nM and 1.0–80 nM and a Limit of Detection (LOD) of 0.045 nM. The proposed biosensor showed a good selectivity toward Ba2+ ions in the presence of other metal ions such as Ag+, Hg2+, K+ and Na+ and was applied to the analysis of natural samples showing appropriate results.

Nucleic acid-based electrochemical biosensor: Recent advances in probe immobilization and signal amplification strategies.

With the increasing importance of accurate and early disease diagnosis and the development of personalized medicine, DNA-based electrochemical biosensor has attracted broad scientific and clinical interests in the past decades due to its unique hybridization specificity, fast response time, and potential for miniaturization. In order to achieve high detection sensitivity, the design of DNA electrochemical biosensors depends critically on the improvement of the accessibility of target molecules and the enhancement of signal readout. Here, we summarize the recent advances in DNA probe immobilization and signal amplification strategies with a special focus on DNA nanostructure-supported DNA probe immobilization method, which provides the opportunity to rationally control the distance between probes and keep them in upright confirmation, as well as the contribution of functional nanomaterials in enhancing the signal amplification. The next challenge of biosensors will be the fabrication of point-of-care devices for clinical testing. The advancement of multidisciplinary areas, including nanofabrication, material science, and biochemistry, has exhibited profound promise in achieving such portable sensing devices. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > Diagnostic Nanodevices Biology-Inspired Nanomaterials > Nucleic Acid-Based Structures.

Development of an ultrasensitive electrochemical biosensor for detection of Agrobacterium tumefaciens in Rosa hybrida L.

Crown gall is one of the destructive diseases of Rosa hybrida L. caused by Agrobacterium tumefaciens. Development of galls leads to an overall weakness, reduction in foliage, water stress, and loss of vigor of the infected plants. We have designed an electrochemical DNA-based biosensor for T-DNA detection. Pencil graphite electrode (PGE) was modified with a nanocomposite including reduced graphene oxide (RGO) and Au nanoparticles. The biosensor sensing element consists of a single-stranded DNA (ss-DNA) from the tms2 gene which binds to thiol gold nanoparticles (GNPs). Under the optimal experiments, the proposed biosensor displayed a wide linear range from 1.0 × 10−13 to 1.0 × 10−9 M with a limit of detection (LOD) of 0.87 × 10−13 M towards target DNA. Experimental analysis on real samples confirmed its application. The proposed biosensor showed an easy and quick detection with high efficiency which was specific to T-DNA contamination of A. tumefaciens, and exhibited high reproducibility and stability.

Development of an advanced DNA biosensor for pathogenic Vibrio cholerae detection in real sample

Due to the epidemics of emerging microbial diseases worldwide, the accurate and rapid quantification of pathogenic bacteria is extremely critical. In this work, a highly sensitive DNA-based electrochemical biosensor has been developed to detect Vibrio cholerae using gold nanocube and 3-aminopropyltriethoxysilane (APTES) modified glassy carbon electrode (GCE) with DNA carrier matrix. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) experiments were performed to interrogate the proposed sensor at each stage of preparation. The biosensor has demonstrated high sensitivity with a wide linear response range to target DNA from 10−8 to 10−14 (R2= 0.992) and 10−14 to 10−27 molL−1 (R2= 0.993) with a limit of detection (LOD) value of 7.41 × 10−30 molL−1 (S/N = 5). The biosensor also exhibits a selective detection behavior in bacterial cultures that belong to the same and distant genera. Moreover, the proposed sensor can be used for six consecutive DNA assays with a repeatability relative standard deviations (RSD) value of 5% (n = 5). Besides, the DNA biosensor shows excellent recovery for detecting V. cholerae in poultry feces, indicating that the designed biosensor could become a powerful tool for pathogenic microorganisms screening in clinical diagnostics, food safety, and environmental monitoring.

Emerging DNA-based multifunctional nano-biomaterials towards electrochemical sensing applications.

DNA is known to be ubiquitous in nature as it is the controlling unit for genetic information storage in most living organisms. Lately, there has been a surge in studies relating to the use of DNA as a biomaterial for various biomedical applications such as biosensing, therapeutics, and drug delivery. The role of DNA as a bioreceptor in biosensors has been known for a long time. DNA-based biosensors are gradually evolving into highly sophisticated and sensitive molecular devices. The current realization of DNA-based biosensors embraces the unique structural and functional properties of DNA in the form of a biopolymer. The interesting properties of DNA, such as self-assembly, programmability, catalytic activity, dynamic behavior, and precise molecular recognition, have led to the emergence of innovative DNA assembly based electrochemical biosensors. This review article aims to cover the recent progress in the field of DNA-based electrochemical (EC) biosensors. It commences with an introduction to electrochemical biosensors and elucidates the advantages of integrating DNA-based materials into them. Besides this, we discuss the principles of EC biosensors based on different types of DNA-based materials. The article concludes by highlighting the outlook and importance of this interesting field for biomedical developments.

Label-Free DNA Biosensor Using Modified Reduced Graphene Oxide Platform as a DNA Methylation Assay.

This work reports the use of modified reduced graphene oxide (rGO) as a platform for a label-free DNA-based electrochemical biosensor as a possible diagnostic tool for a DNA methylation assay. The biosensor sensitivity was enhanced by variously modified rGO. The rGO decorated with three nanoparticles (NPs)—gold (AuNPs), silver (AgNPs), and copper (CuNPs)—was implemented to increase the electrode surface area. Subsequently, the thiolated DNA probe (single-stranded DNA, ssDNA−1) was hybridized with the target DNA sequence (ssDNA-2). After the hybridization, the double-stranded DNA (dsDNA) was methylated by M.SssI methyltransferase (MTase) and then digested via a HpaII endonuclease specific site sequence of CpG (5′-CCGG-3′) islands. For monitoring the MTase activity, differential pulse voltammetry (DPV) was used, whereas the best results were obtained by rGO-AuNPs. This assay is rapid, cost-effective, sensitive, selective, highly specific, and displays a low limit of detection (LOD) of 0.06 U·mL−1. Lastly, this study was enriched with the real serum sample, where a 0.19 U·mL−1 LOD was achieved. Moreover, the developed biosensor offers excellent potential in future applications in clinical diagnostics, as this approach can be used in the design of other biosensors.

Evaluation of antioxidant activity of chlorogenic acids and coffee extracts by an electrochemical DNA-based biosensor

Sensitivity of dsDNA structure towards OH radicals as the pro-oxidants has been utilized as the detection principle of an analytical procedure applied for the first time to the evaluation of antioxidant activity (AOA) of 6 chlorogenic acids (CGAs) and extracts of 10 coffees. A nanostructured electrochemical DNA-based biosensor was prepared using a commercial electrode assembly and treated in the DNA cleavage agent formed by the Fenton type reaction. An addition of CGAs and aqueous coffee extracts significantly diminishes the degree of DNA degradation determined using cyclic voltammetry (CV) with the redox indicator [Fe(CN)6]3−/4−. The AOA decreases in order caffeic acid, CFA, >caffeoylquinic acids, CQAs, >dicaffeoylquinic acids, diCQAs, exhibiting the relative portion of survived DNA of about 71%, 70% and 69%, respectively, and of about 72% for C. robusta, Cherry, India (green bean) to 49% for Nescafé Espresso. Mechanisms of antioxidative properties are discussed.